Freezing of Nonwoody Plant Tissues
نویسندگان
چکیده
منابع مشابه
Tandem high-pressure freezing and quick freeze substitution of plant tissues for transmission electron microscopy.
Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-friendly technique. Traditional sample preparation for TEM used chemical fixatives to preserve cellu...
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Introduction Since plants are immobile, they have to survive the various stresses that they encounter. Freezing is one of the severest stresses as it causes ice formation, dehydration and cell deformation. As plant tissues contain a large amount of water, the behaviour of cell water at subfreezing temperatures is a key to tissue survival. To withstand freezing, cold-hardy plants have developed ...
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When investigating the freezing behaviour (by thermal analysis) of the glycerol-extracted adductor muscle of Mytilus edulis it was observed that the temperature of ice formation in the muscular tissue was up to 1.5 degrees C lower than the freezing point of the embedding liquid, a 0.25 N KCl solution with pH = 4.9 with which the tissue had been equilibrated prior to the freezing experiment. A s...
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This paper contains a few comments on the physical analysis of the factors controlling the rate of cooling, and the relation of the rate of cooling to ice crystal formation which has been carried out in connection with the electron microscopic work described by Dr. C-ersh at this conference. Of the many factors which influence the rate of cooling, by far the most important is the size of the sa...
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Methods Homogenization and extraction with organic solvents was done in one microcentrifuge tube and accelerated by crushing the plant material in a vibration mill. The extracts from minute amounts of fresh plant material were immediately purified using a solid-phase extraction (C18, C8, OasisTM HLB cartridges)in combination with ion-exchange and/or immunoaffinity purification. A fast chromatog...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1974
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.53.5.705